Ctcf knockout 293t cell line

Author: rnkp

August undefined, 2024

WebDetection of human CTCF by western blot of immunoprecipitates. Samples: Nuclear Extract (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells.Antibodies: Affinity purified rabbit anti-CTCF antibody A300-543A (lot A300-543A-4) used for IP at 6 µg per reaction. CTCF was also immunoprecipitated by rabbit anti-CTCF antibody A300-542A. WebSep 13, 2024 · Here, we analyze the genome-wide effect of CTCF in a zygotic ctcf knockout mutant in zebrafish that allows us to monitor the effect of CTCF loss of function during embryonic development. CTCF ...

293TT - 293TT - CCR Wiki - National Cancer Institute

WebNov 30, 2024 · 2. Results. We used shRNA knockdown to model the cellular consequences of reduced CTCF expression in K562 cells. Western blots showed that CTCF protein expression was significantly knocked down by ~80% in the presence of doxycycline (dox) in sh.CTCF K562 cells compared to non dox-treated cells and sh.control cells (Figure 1 … WebThe AAVpro 293T Cell Line is a subclone of the transformed human embryonic kidney cell line HEK 293, which is highly transfectable and supports high levels of viral protein expression. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen. When combined with the AAVpro Helper Free System (Cat. No. 6230), these ... north america birds of prey

CTCF is a barrier for 2C-like reprogramming - Nature

WebCTCF knockout reveals an essential role for this protein during the zebrafish development Authors Francisco Carmona-Aldana 1 , Cecilia Zampedri 2 , Fernando Suaste-Olmos 3 , … WebFeb 17, 2024 · Also, in this cell line, STAG1 stability on chromatin was significantly reduced following CTCF depletion (Figure 3—figure supplement 3A and B). ... , as we had previously observed in primary Ctcf ‘knockout’ MEFs arrested in G0; see Figure 1A in Busslinger et al. (2024). This indicates that CTCF is also required for cohesin acetylation in ... Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific immortalised cell line derived from a spontaneously miscarried or aborted fetus or human embryonic kidney cells grown in tissue culture taken from a female fetus in 1973. HEK 293 cells have been widely used in cell biology research for many years, because of their r… north america blackout

Acute depletion of CTCF rewires genome-wide chromatin …

293T - CRL-3216 ATCC

WebFeb 10, 2024 · To generate the CTCF C-terminal FLAG-tagged cell line, E14TG2a mouse ESCs were targeted with sgRNA in SpCas9-2AGFP vector (Addgene, PX458) and single-stranded donor oligonucleotide at the Ctcf ... WebApr 26, 2015 · I have problems with HEK293 cell line and its IC50 value. In literature, 200-500 ng/ml is suggested as g418 concentration. To be convinced, I performed kill curve (in 96-well plate, 600-60 and 800 ... how to repair a broken gas lineWebJul 20, 2016 · The CCCTC-binding factor (CTCF) is the main insulator protein described in vertebrates. It plays fundamental roles during diverse cellular processes. CTCF gene … how to repair a broken coffee mug

"WebNov 14, 2024 · Generation of HEK293 GLUL knockout (KO) cells. (a) Schematic of the three GLUL isoforms.HEK293 wildtype (WT) cells were transfected with vectors encoding Cas9 and two gRNAs targeting the first ... " - Ctcf knockout 293t cell line

Ctcf knockout 293t cell line

CTCF is a barrier for 2C-like reprogramming - Nature

WebSynthetic peptide within Human CTCF aa 700-800. The exact sequence is proprietary. (Peptide available as ab209492) Positive control WB: HeLa and 293T cell lysates, … WebJan 5, 2024 · A reduction of CTCF expression is known to develop distinct clinical features. Furthermore, conditional knock out (cKO) study revealed reactive gliosis of astrocytes …

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WebA band was observed at 12 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in B2M knockout cell line ab266828 (knockout cell lysate ab256845). To generate this image, wild-type and B2M knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose … Web1. Almost 50% of cells were alive even after 6ug/mL puromycin treatment. 2. HEK293 became suspension cells after puromycin treatment. Some details: At first, I tried the puromycin concentration (2 ...

WebFeb 23, 2010 · Although CTCF occupies these sites in both erythroid K562 cells and fibroblast 293T cells, the long-range interaction frequencies among the sites are highly cell type specific, revealing a more densely clustered organization in the absence of globin gene activity. Both CTCF and cohesins are required for the cell-type-specific chromatin ... WebJan 11, 2024 · Here the authors provide further support for the role of MyoD in 3D genome architecture in muscle stem cells by comparing MyoD knockout and wild-type mice. ... CTCF, and 1 × 10 7 cells and 5 µg ...

Web293T is an epithelial-like cell that was isolated from the kidney of a patient. This cell line was deposited by Stanford University and can be used in vaccine development. Product … WebJun 22, 2024 · Mice and germ cell preparations. Boris and Ctcf knockout mouse models have been previously described 22,32. ... K562 BORIS knockout cell line was generated previously by us 33.

WebAug 24, 2024 · Moreover, CTCF is indispensable for genome-wide TAD and intra-TAD loop formation in a CTCF acute depletion cell model as well as other gene knockout models [17,18,19,20,21]. Despite a global reduction of chromatin interactions upon CTCF loss, its observed effect on mRNA expression by RNA-seq is not dramatic [ 19 , 22 ].

WebHuman CD47 knockout HEK-293T cell line (ab266324) Datasheet SDS Submit a review Submit a question $3,780.00 Product size 1 × 10 6 cells/vial Add to basket Order now … how to repair a broken fingernailWeb1. Thaw the vial in 37°C water bath for approximately 1-2 minutes. 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g ... north america bmwWebNK1R receptor is absent in the 293T ,whereas 293T cells are resistant to SP even at milimolar concentrations. - competent to replicate vectors carrying the SV40 region of replication. - It gives ... north america bmw financialWebJun 22, 2024 · CTCF is a key organizer of the 3D genome. Its specialized paralog, BORIS, heterodimerizes with CTCF but is expressed only in male germ cells and in cancer states. Unexpectedly, BORIS-null mice ... north america blankWebJan 5, 2024 · CCCTC-binding factor (CTCF) is a transcription factor that is involved in organizing chromatin structure. A reduction of CTCF expression is known to develop distinct clinical features. Furthermore, conditional knock out (cKO) study revealed reactive gliosis of astrocytes and microglia followed by age-dependent cell death in the excitatory neurons … north america blank physical mapWebJul 17, 2024 · Exogenous overexpression of target genes in both general and specific cell types is important for mechanistic studies of gene function. Here, we provide a step-by-step protocol for cell culture, plasmid transfection in HEK293T, and adenoviral infection in C2C12 cells for gene overexpression in vitro, using MG53 as an example. This protocol enables … north america bodies of water quiz north america bmw customer service