E2f antibody

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August undefined, 2024

WebFeb 26, 2024 · The following secondary antibodies were used: HRP-linked anti-mouse IgG antibody (AP124P, Millipore, Burlington, MA, USA), and HRP-linked anti-rabbit IgG antibody (AP132P, Millipore). The blotted proteins were visualized by using Western ECL solution (34080, Pierce) and images were obtained by using chemiluminescent image … WebAll lanes : Anti-E2F8 antibody (ab109596) at 0.1 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 5 µg Lane 4 : HeLa whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 94 kDa Exposure time: 3 ...

E2F–HDAC complexes negatively regulate the tumor suppressor …

WebAug 18, 2024 · E2F1 Antibody (KH95) is a mouse monoclonal IgG 2a κ E2F1 antibody, cited in 746 publications, provided at 200 µg/ml; raised against amino acids 342-386 of … WebApr 14, 2024 · Inclusion of CXCR3 neutralizing antibodies reduced CD8 + T-cell adhesion to MGEC monolayers in the context of cytokine stimulation ± ... Dnmt1 is a known E2F target gene upregulated in mitotic ... lightning strike sound effects

E2F1 Antibody (KH95) SCBT - Santa Cruz Biotechnology

WebMar 21, 2024 · GeneCards Summary for E2F1 Gene. E2F1 (E2F Transcription Factor 1) is a Protein Coding gene. Diseases associated with E2F1 include Retinoblastoma and … WebE2f antibody LS-C186487 is an unconjugated goat polyclonal antibody to drosophila E2f (aa480-493). Validated for IF and Peptide-ELISA. Target. Drosophila E2f. Synonyms. E2f. See All E2f Antibodies. Host. Goat. Reactivity. Drosophila (tested or 100% immunogen sequence identity) Clonality ... WebMar 19, 2002 · The E2F family of transcription factors is thought to be a key regulator of cell growth control. To date, six different E2Fs have been identified, E2F1 to -6, each of which can heterodimerize with DP1 or DP2 to form 12 different DNA binding transcriptional regulators ( 1 – 3 ). lightning strike victims

The E2F transcriptional network: old acquaintances with new faces

Negative regulator of E2F transcription factors links cell cycle ... - PNAS

WebE2F1 Antibodies. Antibodies that detect E2F1 can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, … WebE2F is a group of genes that encodes a family of transcription factors (TF) in higher eukaryotes. Three of them are activators: E2F1, 2 and E2F3a. Six others act as suppressors: E2F3b, E2F4-8. All of them are involved in the cell cycle regulation and synthesis of DNA in mammalian cells. lightning strike victims picsWebMA5-17070 targets E2F1 in FACS, IHC, pep-ELISA, and WB applications and shows reactivity with Human and Mouse samples. The MA5-17070 immunogen is purified recombinant fragment of human E2F1 (amino … peanut foam massage roller

"WebAntibody: Immunogen: E2F1 fusion protein Ag2813: Full Name: E2F transcription factor 1: Calculated molecular weight: 437 aa, 47 kDa: Observed molecular weight: 55 kDa: … " - E2f antibody

E2f antibody

A critical role of E2F transcription factor 2 in ... - Nature

WebSep 11, 2024 · The transcription factor, E2 promoter binding factor 1 (E2F-1), exhibits tumor promoter or suppressive functions depending on cellular type and tissue context, but its role in the progression and development of colorectal carcinogenesis was largely unknown. Here, using a tamoxifen-inducible PTEN knockout mouse model, we have demonstrated … WebJan 30, 2001 · The transfected cells were labeled with [35 S]methionine and then immunoprecipitated with anti-E2F antibodies (to confirm expression of the E2F proteins) or an anti-HA antibody (to detect RYBP and associated proteins). Although E2F6 was readily detected in the RYBP immunoprecipitation, ...

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WebE2F-7 antibody; E2f7 antibody; E2F7_HUMAN antibody; FLJ12981 antibody; Transcription factor E2F7 antibody; see all. Images. Immunoprecipitation - Anti-E2F7 antibody (ab245655) E2F7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded). WebThe sample was probed with a E2F7 polyclonal antibody (Product # PA5-68911) using a primary antibody dilution of 1.0 µg/mL. Fig. 4 Biological role of miRNA-302a/d and E2F7 in HCC in vitro and in vivo. qRT-PCR ( a ) and Western blot ( b ) to quantify E2F7 levels in adherent and tumor spheres of HepG2 and Huh7 cells after 3 and 7 days cultured ...

WebListed below are anti-E2f antibodies from multiple suppliers. E2f is a reported alias name for the human gene E2F1, or 'E2F transcription factor 1'. The 437-amino acid protein is a member of the E2F/DP family. The cellular localization is predicted to be nuclear. WebE2F-7 antibody; E2f7 antibody; E2F7_HUMAN antibody; FLJ12981 antibody; Transcription factor E2F7 antibody; see all. Images. Immunoprecipitation - Anti-E2F7 …

WebAnti-idiotype Antibody Services New! Neoantigen Peptide Service New! CRISPR sgRNA Services; Gene Synthesis; mRNA Synthesis New! ... E2F transcription factors control the expression of genes involved in a variety of essential cellular processes and consequently their activity needs to be tightly regulated. Protein-protein interactions are ... WebBrand: BD Pharmingen™ Reactivity: Human (QC Testing), Mouse, Rat (Tested in Development) Isotype: Mouse IgG2a Immunogen: Human E2F-1 recombinant protein aa. 1-437 Application: Western blot (Routinely Tested), Immunoprecipitation (Reported) Target Molecular Weight: 60 kDa Concentration: 0.5 mg/ml RRID: AB_395308

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WebE2F1 Antibodies. (E2F Transcription Factor 1 (E2F1)) The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. peanut food groupWebThe E2F and Rb genes are expressed in a cell cycle-dependent manner. E2F family members bind E2F sites as heterodimers with members of the DP family (56, 57). … peanut food allergy symptomsWebApr 2, 2024 · The promoter fragments containing E2F consensus motif of E2F target genes (MCM, ORC1B, CDC6, and ORC3) were quantified, and the percentages of coimmunoprecipitated DNA by antibody relative to the input DNA were shown. Actin7 and CYCB1:1 were used as negative controls. Data represent mean ± SE of three biological … peanut food challenge