Psicheck-2 promega

Author: cemr

August undefined, 2024

WebThe psiCHECK™-2 Vector contains a second reporter gene, firefly luciferase, and is designed for endpoint lytic assays. Introduction of firefly luciferase in the psiCHECK™-2 Vector … http://www.labbase.net/Supply/SupplyItems-4229347.html

Programmable siRNA pro-drugs that activate RNAi ... - ScienceDirect

WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Renilla luciferase is used as the primary reporter gene, and the gene of interest is cloned into a multiple cloning region located ... barakallah dua

Addgene: psiCHECK-2-XNS

WebpsiCHECK™-2 Vector. Luciferase reporter vector for monitoring RNA interference effects. C8021. GloSensor™ Technology. Live-cell biosensors for cAMP, cGMP or protease activity. E1290, E1291, E2301, E1171 WebJan 3, 2024 · a PsiCheck-2 dual-luciferase reporter (Promega) carrying the siRNA target in the 3′ UTR of Renilla luciferase, and (2) a pBlueScript 34 vector with a polymerase III (Pol III) promoter expressing short RNA transcripts that had different biomarker sequences 35 ( … WebThe psiCHECK™-2 Vector is designed to provide quantitative and rapid optimization of RNA interference (RNAi). The vector enables monitoring of changes in expression of a target gene fused to a reporter gene. Renilla … barakallah fi khair

Psicheck 2 Luciferase Reporter Vector Promega Bioz

WebNational Center for Biotechnology Information WebFeb 21, 2024 · In the psiCHECK-2 vector, Renilla is expressed under SV40_promotor/enchancer and is a test luciferase, whereas Firefly is expressed under HSV-TK promoter and is an endogenous control. Therefore,... barakallah arabicWebThe psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to the reporter gene. barakallah calligraphy

"WebAug 13, 2024 · The 3′UTR of CREBL2 and PCBP2 were sub-cloned into the psiCHECK-2 vector (Promega, USA) downstream of the Renilla luciferase reporter gene. Flanking sequence on each side was synthetized with a short extension containing cleavage sites for XhoI (CTCGAG) and NotI (GCGGCCGC), and the primer sequences are shown in Table … " - Psicheck-2 promega

Psicheck-2 promega

Programmable siRNA pro-drugs that activate RNAi activity in ... - Cell

WebpsiCHECK-2 Vector, 20 µg, Promega Supplier: Promega psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference assays. Vectors for Optimizing RNA Interference Assays WebYou've created a Promega.com account. Check your inbox to complete email verification. Note: You will not be able to access your account until your email is verified. Close Existing Account Found. ... psiCHECK™-2 Vector C8021 PDF (190 KB) – English (United ...

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WebDual Luciferase Reporter Vector Psicheck 2, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … WebpsiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Total vector size (bp) 6302 Vector type Mammalian Expression, Luciferase ; microRNA activity Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert

WebCotransfection of porcine pre-miR-155 or control and porcine OLFML3 3'UTR-derived psiCHECK-2 construct or mutant in PK15 and PIEC cells. Renilla activity at 48 h post-transfection shows a significant decrease in normalized values than … WebpsiCHECK™-2: miR-21 Biosensor No light Figure 1. Detection of miR-21 activity using the psiCHECK™-2 Vector. miR-21 target sequence was cloned into the multiple cloning …

WebNov 3, 2024 · psiCHECK™-2 Vector（C8021）最初是为验证 siRNA 靶标设计的。该载体用海肾萤光素酶（hRluc）作为主报告基因；萤火虫萤光素酶（hluc+）则作为内参报告基因，用于进行归一化处理。目的基因被克隆至海肾萤光素酶翻译终止密码子下游的多克隆位点。由合成的 siRNA 或体内表达的 shRNA 引发的针对目的基因的 RNAi 过程，导致融合 … WebpsiCHECK-2 Vector, 20 µg, Promega Supplier: Promega psiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter …

WebpsiCHECK2 (Search Vector Database) Backbone manufacturer Promega Backbone size (bp) 6273 Modifications to backbone Insertion of a SalI/NotI/XhoI linker between the NotI and XhoI site Vector type Mammalian Expression Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number

WebAnalyze Sequence: psiCHECK-2. Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn about the latest plasmid technologies and research tools. Subscribe. Contact Addgene. Have questions about your order, deposit, or a plasmid? barakallah fi umrik yesusWebThe psiCheck-2 vector contains two luciferase genes, renilla as the experimental reporter while firefly the control reporter. Renilla is the experimental reporter as you can clone in … barakallah fi umrik artinyahttp://www.honorgene.com/uploads/files/202404/5ea518d17dd8f.pdf barakallah fi umrik bahasa arab